Assay characteristics:
| Cat.No.: | BI-20403 |
| Method: | Sandwich ELISA, HRP/TMB, 12x8-well strips |
| Sample type: | Serum, plasma (EDTA, heparin, citrate) |
| Standard range: | 0-20 pmol/l (6 serum based standards) |
| Standard points: | 0/1.25/2.5/5/10/20 pmol/l |
| Control: | 1 serum based control |
| Sample size: | 20 μl / well |
| Incubation time: | 4 h / 1 h / 30 min |
| Unit conversion: | 1 pg/ml = 0.05 pmol/l (MW: 19.9 kDa) |
Sensitivity:
LOD: 0.07 pmol/l (0 pmol/l + 3 SD); LLOQ: < 0.08 pmol/l
Precision:
Intra-assay (n=5) ≤ 3%, Inter-assay (n=12) ≤ 5%
Spike/Recovery:
The mean recovery of recombinant OPG in serum and EDTA plasma is between 93-108%.
The mean recovery of recombinant OPG in heparin - and citrate plasma is between 82-109%.
Dilution linearity:
The dilution linearity of endogenous OPG in serum samples (n=3) is between 79-102%.
The dilution linearity of recombinant OPG in serum samples (n=8) is between 80-116%.
Cross reactivity:
The assay does not cross react with rat or mouse samples.
Values from apparently healthy individuals:
Median (serum, n=60): 2.7 pmol/l.
It is recommended to establish the normal range for each laboratory.
Principle of the assay:
Manual ELISAs - easily adaptable for automation!