Assay characteristics:
| Cat.No.: | BI-20413 |
| Method: | Sandwich ELISA, HRP/TMB, 12x8-well strips |
| Sample type: | Serum |
| Standard range: | 0-160 pmol/l (6 serum based standards) |
| Standard points: | 0/10/20/40/80/160 pmol/l |
| Control: | 1 serum based control |
| Sample size: | 20 μl / well |
| Incubation time: | 2 h / 1 h / 30 min |
| Unit conversion: | 1 pg/ml = 0.039 pmol/l (MW: 25.8 kDa) |
Specificity:
oligeomeric forms of natural and recombinant DKK-1.
Sensitivity:
LOD: 1.7 pmol/l (0 pmol/l + 3 SD)
Precision:
Intra-assay (n=5) ≤ 3%, Inter-assay (n=9) ≤ 3%
Spike/Recovery:
The mean recovery of recombinant DKK-1 in human serum samples (n=8) is 92% (spike: 40 pmol/l) and 83% (spike: 80 pmol/l).
Dilution linearity:
Average recovery of expected DKK-1 after a 1+1; 1+3; 1+7 dilution in serum (n=4) is 109%; 104%; 100%
Cross reactivity:
Human samples only, no cross reactivity with other species. No cross reactivity or interference with recombinant human DKK-4, Kremen-1, Kremen-2, or LRP-6 was observed.
Values from apparently healthy individuals:
Median (serum, n=51) = 34 pmol/l
It is recommended to establish the normal range for each laboratory.
Principle of the assay:
Manual ELISAs - easily adaptable for automation!