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英文名稱：Bone Slices(pack 50 slices)

骨切片使用于采用傳統(tǒng)染色法或Crosslaps® for culture ELISA法對破骨細(xì)胞骨吸收進(jìn)行精確的體外評估

Bone slices* are accurate in vitro assessment of osteoclastic bone resorption either by traditional staining or by use of the CrossLaps® for Culture ELISA assay.

Bone is a dynamic tissue that is remodelled through life. It is believed that special factors are present in bone, which may affect the activity of osteoclast. Thus at IDS we use high quality bone, not dentine, for all our research.

Each batch of bone slices is tested for quality by resorption assays for verification of osteoclastic ability to make pits on these exact bone slices. Subsequently, the supernatant is measured by the Crosslaps® for Culture ELISA kit and only if both tests are positive the bone slices are accepted for further resorption assays.

Assay Details:
 
Storage
    The bone slices are stored in 70% ethanol at 4°C. However, for shorter time periods and during transport room temperature is adequate for retaining the quality of the bone slices.

Handling
    We recommend that our bone slices are washed 3 times in 10% serum containing medium before they are transferred to the 96 well plates.

Culture
    Usually resorption experiments lasts 72 hours, however already after 16 or 24 hours after addition compound to osteoclast cultures a significant differences are observed. Even after 8 hours a significant difference can be observed in optimised assays.