The Serum CrossLaps ® ELISA is used for assessment of bone resorption. The assay detects collagen type I fragments generated during osteoclastic bone resorption and employs monoclonal antibodies recognizing C-telopeptide fragments of collagen type I a1 chains containing the epitope Glu-Lys-Ala-His-Asp- ƒÅ¸-Gly-Gly-Arg in an isomerised form.
· Able to monitor changes in bone resorption during hormone replacement therapy (HRT) and treatment with bosphosphonates with both serum and plasma samples
· Additional test includes being used for prediction of skeletal response to anti-resorptive therapy.
· Type I collagen accounts for more than 90%of the organic matrix of bone and is synthesised primarily in bone(1). During renewal of the skeleton, Type I collagen is degraded, and small peptide fragments are excreted into the bloodstream. These fragments can be measured by SerumCrossLaps ® ELISA. The measurements of the specific degradation products of Type I collagen in both urine(2)and serum(3)by a competitive CrossLaps ® have been reported.
· The SerumCrossLaps ® ELISA assay has been reported as useful for follow up of anti-resorptive treatment of patients with metabolic bone diseases(3-17).
|
Code |
AC-02F1 |
|
Format |
96 Wells |
|
Methodology |
ELISA |
|
Number of Tests per Kit |
40 in duplicate |
|
Standards |
6 |
|
Controls |
2 |
|
Specimen Type |
Serum or plasma |
|
Specimen Volume |
50 µL |
|
Detection Limit |
0.010 ng/ml |
|
Specificity |
C-Terminal telopeptide a1 chain of type I collagen (CTX-I) in human, monkey, bovine, horse, sheep, goat, pig, dog, cat, elephant, chicken |
|
Assay Range |
approx 0 - 2.5 ng/mL |
|
Precision |
CV's within run <6%; CV's between runs <10% |
|
Total Assay Time |
3 Hours |
|
Shelf Life |
12 Months from point of manufacture |
|
Shaker Speed |
300 rpm |
|
Intended Use |
For IVD Use |
1. Bjarnason et al., Bone 26 :561-569 (2000)
2. Buclin et al., J Bone Miner Res 17 :8-1478 (2002/
3. Christgau et al., Bone 26 :505-511 (2000)
4. Delmas Osteoporos Int 11 :S66-S76 (2000)
5. Delmas et al., Bone 26 :553-560 (2000)
6. Fink et al., Osteoporosis Int 11, 295-303 (2000)
7. Ravn et al., Bone 30, 320-324 (2002)
8. Ravn et al., Bone 24, 237-244 (1997)
9. Reginster et al., Calcif Tissue Int 69, 130-137 (2001)
10. Rosen et al., Calcif Tissue Int 66, 100-103 (2000)
