NT-proBNP ELISA
貨號(hào): |
SK-1204 |
方法學(xué): |
Sandwich ELISA, HRP/TMB, 12x8-well strips |
樣本類型: |
Serum and EDTA plasma |
標(biāo)準(zhǔn)品范圍: |
0-640 pmol/l (5 serum based standards) |
標(biāo)準(zhǔn)品濃度: |
0/10/40/160/640 pmol/l |
質(zhì)控品: |
1 serum based control |
樣本量: |
50 μl / well |
孵育時(shí)間: |
3 h / 30 min |
單位換算: |
1 pg/ml = 0.118 pmol/l (MW: 8.475 kDa) |
靈敏度:
LOD: 3 pmol/l (0 pmol/l + 3 SD)
精密度:
Intra-assay (n=3) ≤ 3%, Inter-assay (n=8) ≤ 7%
回收率:
The mean recovery of recombinant NT-proBNP in serum samples (n=4) is 104%, in
EDTA plasma samples (n=4) is 94%.
線性: Dilution
linearity for serum samples (n=3) is between 79-86% for endogenous NT-proBNP
(1-76) and 112-119% for recombinant NT-proBNP (1-76).
Dilution linearity for EDTA plasma samples (n=2) is between 83-84% for
endogenous NT-proBNP (1-76) and 83-86% for recombinant NT-proBNP (1-76).
交叉反應(yīng):
The assay works with human samples only.
參考范圍(健康人群):
Median (serum, n=70): 5.8 pmol/l.
Median (EDTA plasma, n=28): 5.6 pmol/l.
推薦實(shí)驗(yàn)室建立自己的參考值。
本產(chǎn)品僅供科研使用