可溶性總神經(jīng)纖毛蛋白-1檢測(cè)試劑盒(酶聯(lián)免疫法)
Total Soluble Neuropilin-1 ELISA
全球首家推出由表位定位特異性抗體定量檢測(cè)
nNrp-1在神經(jīng)系統(tǒng)中與其配體 semaphorins (信號(hào)素）結(jié)合而在軸突中起作用， Nrp-1， Nrp-2為 Sema受體并且僅與III型Sema結(jié)合。
應(yīng)用領(lǐng)域：骨學(xué)，腫瘤學(xué)，腎臟學(xué)，心血管。
試劑盒信息
試劑盒信息
貨號(hào) |
BI-20409 |
方法學(xué) |
夾心 ELISA, HRP/TMB, 12x8人份測(cè)試 |
樣本類型 |
血清、血漿(EDTA, 肝素,枸櫞酸鈉)、細(xì)胞培養(yǎng)液 |
標(biāo)準(zhǔn)品范圍 |
0-12 nmol/l (7個(gè)標(biāo)準(zhǔn)品） |
標(biāo)準(zhǔn)品 |
0/0.37/0.75/1.5/3/6/12 nmol/l |
質(zhì)控品 |
2個(gè) |
樣本量 |
10 μl /測(cè)試 |
孵育時(shí)間 |
30 min/2h/1h/30min |
單位換算 |
1ng/ml = 0.014nmol/l (MW: 69.7 kDa) |
靈敏度 |
LOD: 0.09 nmol/l (0 pmol/l + 3 SD); LLOQ: 0.09nmol/l |
精密度 |
批內(nèi)差 (n=6) ≤ 11%, 批間差 (n=12) ≤ 10% |
健康人群參考范圍 |
Median 血清( n=24): 2.0nmol/l. Median EDTA 血漿(n=24): 1.7nmol/l. Median 肝素 血漿(n=24): 1.7nmol/l. Median枸櫞酸鈉血漿(n=24): 1.9nmol/l. |
非人屬種Nrp-1 |
猴子，豬，大鼠及小鼠與人Nrp-1同源性超過(guò)90%，本試劑并未對(duì)非人屬種樣本做驗(yàn)證，但是大鼠及小鼠的加標(biāo)回收率以及線性數(shù)據(jù)表現(xiàn)合格。
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參考文獻(xiàn)
1.Neuropilin Functions as an Essential Cell Surface Receptor. HF Guo and CW Vander Kooi, J Biol Chem, 2015; 290(49):29120-29126.
2.Neuropilin signalling in angiogenesis. Koch S, Biochem Soc Trans, 2012; 40(1):20-25.
3. Characterization of neuropilin-1 structural features that confer binding to semaphorin 3A and vascular endothelial growth factor 165. Gu C et al., J Biol Chem, 2002; 277(20): 18069-18076.
4. Multifaceted Role of Neuropilins in the Immune System: Potential Targets for Immunotherapy. Roy S et al., Front Immunol, 2017; 10 (8): 1228.
5.Neuropilin 1 expression in human aortas, coronaries and the main bypass grafts. Alattar M et al., Eur J Cardiothorac Surg, 2014; 46(6): 967-973.
6. Role of Neuropilin-1 in Diabetic Nephropathy. Bondeva T et al., J Clin Med, 2015; 4(6): 1293-1311.
7.Neuropilins: role in signalling, angiogenesis and disease. Zachary I, Chem Immunol Allergy, 2014; 99: 37–70.
8. Neuropilin-1 is upregulated in the adaptive response of prostate tumors to androgen-targeted therapies and is prognostic of metastatic progression and patient mortality. Tse BWC et al., Oncogene, 2017; 36(24): 3417-3427.
9. Dual Function of NRP1 in Axon Guidance and Subcellular Target Recognition in Cerebellum. Telley L et al., Neuron, 2016; 21; 91(6): 1276-1291.
10. Nrp1, a Neuronal Regulator, Enhances DDR2-ERK-Runx2 Cascade in Osteoblast Differentiation via Suppression of DDR2 Degradation. Zhang Y et al., Cell Physiol Biochem, 2015; 36(1): 75-84.
11.Neuropilins: structure, function and role in disease. Pellet-Many C et al., Biochem J, 2008; 411(2): 211-226.
12. Neuropilin-1 as Therapeutic Target for Malignant Melanoma. G Graziani1 and PM Lacal, Front Oncol, 2015; 5: 125.
13. CPMP/ICH/381/95 ICH Topic Q2 (R1) „Validation of Analytical Procedures: Text and Methodology” including: ICH Q2A “Text on Validation of Analytical Procedures” ICH Q2B “Validation of Analytical Procedures: Methodology”.
14.Neuropilins lock secreted semaphorins ontoplexins in a ternary signaling complex. Janssen BJC et al., Nat struct Mol Biol, 2012; 19:1293-1299.