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艾狄斯®尿膠原降解產(chǎn)物α鏈檢測(cè)試劑盒（酶聯(lián)免疫法）  本產(chǎn)品僅供科研使用

ALPHA CROSSLAPS ELISA

艾狄斯®尿膠原降解產(chǎn)物α鏈測(cè)試試劑盒（酶聯(lián)免疫法）(ALPHACrossLaps^® EIA)（簡(jiǎn)稱：αCTX-I檢測(cè)試劑盒）采用酶聯(lián)免疫吸附測(cè)定法定量測(cè)定人尿液中I型膠原C端肽（αCTX-I）的非異構(gòu)化片段。

作為非異構(gòu)化骨膠原降解的指標(biāo)，αCTX-I檢測(cè)試劑盒可用作鑒定乳腺癌和前列腺癌患者的骨轉(zhuǎn)移的輔助工具。

此外，αCTX-I檢測(cè)試劑盒可應(yīng)用于變形性骨炎的診斷及相應(yīng)抗骨吸收治療療效監(jiān)測(cè)的輔助工具。

特征及優(yōu)勢(shì)

ü性能可靠穩(wěn)定，眾多國(guó)際文獻(xiàn)支持

當(dāng)Alpha CrossLaps® (CTX-I)與TRACP 5b水平同時(shí)升高時(shí)，一定是腫瘤骨轉(zhuǎn)移！

比ICTP指標(biāo)更為靈敏，反映病理性骨吸收！非異構(gòu)化骨膠原降解指標(biāo)劑！

背景知識(shí)

n 尿膠原降解產(chǎn)物α鏈作為非異構(gòu)化骨膠原降解產(chǎn)物的指標(biāo)劑，可用于鑒別早期乳腺癌和前列腺癌骨轉(zhuǎn)移患者，也可用于輔助診斷變形性骨炎及監(jiān)測(cè)抗骨吸收治療療法患者。

n尿膠原降解產(chǎn)物α鏈檢測(cè)試劑盒基于高特異性的抗氨基酸序列EKAHDGGR的單克隆抗體制備。為獲得特異信號(hào)，氨基酸序列EKAHDGGR的兩鏈必須相互交聯(lián)。

【檢測(cè)的概述和說(shuō)明】

I型膠原占骨有機(jī)基質(zhì)的90%以上，并主要在骨骼中合成。骨骼更新時(shí)，I型膠原被降解，短肽片段進(jìn)入到尿液。這些片段可采用αCTX-I檢測(cè)試劑盒來(lái)測(cè)定。

據(jù)文獻(xiàn)報(bào)道，本試劑盒有助于評(píng)估變形性骨炎（1）骨吸收及檢測(cè)前列腺癌(2，5) 和乳腺癌(3，4，5，6)骨轉(zhuǎn)移。 

【檢驗(yàn)原理】

αCTX-I檢測(cè)試劑盒基于高特異性的抗氨基酸序列EKAHDGGR的單克隆抗體制備。為獲得特異信號(hào)，氨基酸序列EKAHDGGR的兩鏈必須相互交聯(lián)。將標(biāo)準(zhǔn)、對(duì)照或未知的尿液樣本移入涂有鏈霉親和素的微滴孔中，然后應(yīng)用生物素化抗體和過(guò)氧化物酶偶聯(lián)抗體的混合物。然后，在抗體交叉抗原、生物素化抗體和過(guò)氧化物酶偶聯(lián)抗體之間生成復(fù)合物，該復(fù)合物通過(guò)生物素化抗體與鏈霉親和素表面結(jié)合。在2 – 8℃進(jìn)一步孵化后,洗板并去除多余的洗液。加入顯色底物，用硫酸終止止顯色反應(yīng)。最后測(cè)量吸光度。

【生產(chǎn)廠商】 艾狄斯

參考文獻(xiàn)

1.Alexandersen et al. Non-Isomerized C-Telopeptide fragments are highly sensitive markers for monitoring disease activity and treatment efficacy in Paget′s disease of bone. J Bone Miner Res. 2005 Apr;20(4):588-95.

2.Cloos et al. Investigation of bone diseases using isomerised and racemised fragments of type I collagen. Calcif Tissue Int (2003);72:8-17.

3.Cloos et al. Breast cancer patients with bone metastases are characterised by increasing levels of nonisomerised type I collagen fragments. Breast Cancer Res (2003);5:R103-9.

4.Cloos et al. An immunoassay for measuring fragments of newly synthesized collagen type I produced during metastatic invasion of bone. Cli. Lab. (2004); 50:279-289.

5.Leeming DJ, Koizumi M, Byrjalsen I, Li B, Qvist P, Tanko LB: The relative use of eight collagenous and noncollagenous markers for diagnosis of skeletal metastases in breast, prostate, or lung cancer patients. Cancer Epidemiol Biomarkers Prev 15: 32-8, 2006.

6.Leeming DJ, Delling G, Koizumi M, Henriksen K, Karsdal MA, Li B, Qvist P, Tanko LB, Byrjalsen I: Alpha CTX as a biomarker of skeletal invasion of breast cancer: immunolocalization and the load dependency of urinary excretion. Cancer Epidemiol Biomarkers Prev 15: 1392-5, 2006.

Some publications here about prostate/breast cancer

以下有一些關(guān)于前列腺癌/乳腺癌的文獻(xiàn) （aCTX-I）：

Alpha CTX as a Biomarker of Skeletal Invasion of Breast Cancer

Demonstrates a significant linear association between the number of bone metastases and the urinary levels of alpha CTX in breast cancer patients with metastatic bone disease

http://cebp.aacrjournals.org/content/15/7/1392.long

Markers of bone turnover for the management of patients with bone metastases from prostate cancer

Levels of alpha CTX significantly higher in patients with prostate cancer and bone metastasis than in patients with benign prostatic hyperplasia, patients with prostate cancer without bone metastases and healthy controls

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2374389/pdf/82-6691012a.pdf

The Relative Use of Eight Collagenous and Noncollagenous Markers for Diagnosis of Skeletal Metastases in Breast, Prostate, or Lung Cancer Patients

Levels of alpha CTX-I were elevated in patients with metastases and was more useful in breast and prostate cancers.  Furthermore, alpha CTX-I was able to discriminate lung cancer patients with or without bone metastases

http://cebp.aacrjournals.org/content/15/1/32.long

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